Detection of Histone Acetylation Enzymes
Using conjugated polyelectrolytes for enzyme activity detection (Nov 2019 – Jan 2021)
Advisor: Prof. Chunyan Tan
Developed fluorescent probes based on conjugated polyelectrolytes (CPEs) for detecting histone acetylation-related enzymes, p300 (acetyltransferase) and SIRT1 (deacetylase).
Key Contributions
- Synthesized conjugated polymer PPE-IDA through Sonogashira coupling and characterized spectroscopic properties
- Designed polylysine peptide substrates of acetylation-related enzymes (p300 and SIRT1) and explored their interaction with several conjugated polyelectrolytes
- Explored the optimal catalytic conditions of enzymes
- Utilized peptide-polymer probes to detect p300 and SIRT1 activity