Fluorescence Sensor Array for Amyloid Beta Aggregates
Cross-reactive sensor array for discriminating Aβ42 aggregation states (Jan 2021 – Apr 2022)
| Advisor: Prof. Chunyan Tan | Published: Analytical Chemistry 2022 (First Author) |
Developed a cross-reactive fluorescence sensor array for rapid and accurate discrimination of different aggregation states of amyloid beta 42 (Aβ42) peptide, which is closely associated with Alzheimer’s disease.
Key Contributions
- Prepared different aggregation states of amyloid beta peptide and characterized them using transmission electron microscopy (TEM) and CD spectrometer
- Discriminated different aggregation states using a fluorescence sensor array containing conjugated polyelectrolytes and small molecules, analyzed by PCA and LDA algorithms (Matlab)
- Prepared on-pathway and off-pathway aggregates by adding various stimuli, and discriminated them using the constructed sensor array
- Explored aggregation kinetics using the constructed fluorescence sensor array
Publication
- Zhang, P.; Tan, C. Cross-Reactive Fluorescent Sensor Array for Discrimination of Amyloid Beta Aggregates. Anal. Chem. 2022, 94 (14), 5469–5473.